Journal:

Article Title: Nanobacteria: An alternative mechanism for pathogenic intra- and extracellular calcification and stone formation

doi:

Figure Lengend Snippet: Nanobacteria cultured under SF conditions and their interaction with cells. (A) Light microscopic micrograph. (B) DNA staining of the same area with the modified Hoechst staining method. (C) DIC images of nanobacteria inside a common apatite shelter. (D) A partly demineralized nanobacterial group (A–D, ×860). (E and F) SEM micrographs of nanobacterial dwellings detached from the culture vessel. (Bars = 1 μm.) (G) IIFS of internalized mineralized nanobacteria (white arrows) in 3T6 cells. (H) DNA staining of the same area with standard Hoechst method. (×540.) (I–L) TEM micrographs of intracellular calcifications in 3T6 cells caused by SF nanobacteria. (Bars: I and K = 2 μm; J = 500 nm; L = 200 nm.)

Article Snippet: 3T6 cells (ATCC CCL 96) were cultured on coverslips as described before ( 5 ).

Techniques: Cell Culture, Staining, Modification

Journal:

Article Title: Nanobacteria: An alternative mechanism for pathogenic intra- and extracellular calcification and stone formation

doi:

Figure Lengend Snippet: Examples of extra- and intracellular calcification by nanobacteria. TEM micrograph of cultured nanobacteria (bar = 200 nm) from FBS (A) and a bacterium in a kidney stone after demineralization (B; bar = 50 nm). (C) IIFS of the same kidney stone with anti-nanobacteria mAb. (D and E) von Kossa staining results of 3T6 cells exposed to SF nanobacteria for 24 hr. (F) Negative control. (×270.)

Article Snippet: 3T6 cells (ATCC CCL 96) were cultured on coverslips as described before ( 5 ).

Techniques: Cell Culture, Staining, Negative Control

Journal: FEMS microbiology letters

Article Title: Complex kinase requirements for Chlamydia trachomatis Tarp phosphorylation.

doi: 10.1111/j.1574-6968.2008.01390.x

Figure Lengend Snippet: Fig. 3. Tarp is a substrate of multiple kinases in vivo. (a) SYF, SYF1Src and L929 cells were infected with Chlamydia trachomatis L2 at a MOI of 250 for 20, 40 and 60 min to investigate Tarp phosphorylation (black arrowheads). Tarp phosphorylation is delayed and weakened in SYF cells while phosphorylation is restored nearly to wild-type levels in SYF1Src cells. (b) Quantification of (a). Tarp phosphorylation was measured by band density measurement and Tarp phosphorylation levels were nor- malized to Tarp expression levels. SYF cells show an average reduction of Tarp phosphorylation by c. 40%. Error bars SEM, n = 2.

Article Snippet: Cell lines, bacterial strains and transfection HeLa (ATCC CCL-2) cervix carcinoma epithelial cells, L929 (ATCC CCL-1), SYF (ATCC CRL-2459) and SYF1Src (ATCC CRL 2498) mouse fibroblasts were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 10% fetal calf serum, 2 mM L-glutamine and 1 mM Na-pyruvate.

Techniques: In Vivo, Infection, Phospho-proteomics, Expressing